{"id":85,"date":"2018-01-08T10:15:18","date_gmt":"2018-01-08T10:15:18","guid":{"rendered":"http:\/\/confolab.sav.sk\/ovsb\/?page_id=85"},"modified":"2023-10-05T12:46:27","modified_gmt":"2023-10-05T12:46:27","slug":"bunkove-kultury","status":"publish","type":"page","link":"https:\/\/confolab.sav.sk\/ovsb\/laboratoria\/bunkove-kultury\/","title":{"rendered":"Bunkov\u00e9 kult\u00fary"},"content":{"rendered":"<h2>CO2 inkub\u00e1tor (ESCO), lamin\u00e1rny box pre pr\u00e1cu v rizikovej triede 2 (ESCO), parn\u00fd autokl\u00e1v (IcanClav)&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<button onclick=\"goBack()\">Nasp\u00e4\u0165<\/button> <\/h2>\n<p><script>\t \t \nfunction goBack() { window.history.go(-1); }\t \t \n<\/script><\/p>\n<h3>Kontakt: RNDr. Michal Cagalinec, PhD.<\/h3>\n<h3>Met\u00f3da kr\u00e1tkodobej kultiv\u00e1cie \u010derstvo izolovan\u00fdch kardiomyocytov a kortik\u00e1lnych neur\u00f3nov a ich transfekcia pomocou:<\/h3>\n<ul>\n<li>Lipofectamine2000\u00ae<\/li>\n<li>adenov\u00edrusov<\/li>\n<\/ul>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-1009\" src=\"https:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/DSC_1393_2-293x300.jpg\" alt=\"DSC_1393_2\" width=\"139\" height=\"142\" \/><\/td>\n<td><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-1010\" src=\"https:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/DSC_1105_1-300x247.jpg\" alt=\"DSC_1105_1\" width=\"173\" height=\"142\" \/><\/td>\n<td><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-1012\" src=\"https:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/DSC_1315_2-300x235.jpg\" alt=\"DSC_1315_2\" width=\"181\" height=\"142\" \/><\/td>\n<td><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-1011\" src=\"https:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/DSC_1381_1-300x264.jpg\" alt=\"DSC_1381_1\" width=\"160\" height=\"141\" \/><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Hlavn\u00fdm zameran\u00edm laborat\u00f3ria je \u0161t\u00fadium organel a patofyziologick\u00fdch procesov v myocytoch a neur\u00f3noch s vyu\u017eit\u00edm fluorescen\u010dn\u00fdch indik\u00e1torov a prote\u00ednov a n\u00e1sledn\u00e1 detekcia fluorescencie v STED, konfok\u00e1lnom a fluorescen\u010dnom mikroskope.<\/p>\n<p>Aby sme v myocytoch resp. neur\u00f3noch mohli exprimova\u0165 dan\u00e9 fluorescen\u010dn\u00e9 prote\u00edny, je nutn\u00e9 plazmidy k\u00f3duj\u00face predmetn\u00e9 prote\u00edny prenies\u0165 do cytoplazmy pomocou lipofekcie (Lipofectamine2000\u00ae) alebo adenovir\u00e1lnou transfekciou.<\/p>\n<h3>Moment\u00e1lne pl\u00e1nujeme exprimova\u0165 prote\u00edny:<\/h3>\n<ul>\n<li>MitoKikumeGR1 \u2013 fotokonvertibiln\u00fd prote\u00edn (zelen\u00e1 \u2192 \u010derven\u00e1) cielen\u00fd do mitochondri\u00ed pre \u0161t\u00fadium dynamiky a f\u00fazie mitochondri\u00ed<\/li>\n<li>Perceval \u2013 fluorescen\u010dn\u00fd FRET prote\u00edn (CFP-YFP) pre meranie pomeru ATP\/ADPv cytoplazme myocytov<\/li>\n<li>Chameleon D3cpv \u2013 fluorescen\u010dn\u00fd FRET prote\u00edn (CFP-YFP) pre meranie koncentr\u00e1cie Ca2+ v cytoplazme, mitochondri\u00e1ch a ER\/SR myocytov<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>CO2 inkub\u00e1tor (ESCO), lamin\u00e1rny box pre pr\u00e1cu v rizikovej triede 2 (ESCO), parn\u00fd autokl\u00e1v (IcanClav)&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Nasp\u00e4\u0165 Kontakt: RNDr. Michal Cagalinec, PhD. Met\u00f3da kr\u00e1tkodobej kultiv\u00e1cie \u010derstvo izolovan\u00fdch&#8230;<span class=\"excerpt_more\"><a href=\"https:\/\/confolab.sav.sk\/ovsb\/laboratoria\/bunkove-kultury\/\">\u010d\u00edta\u0165 \u010falej &raquo;<\/a><\/span><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":18,"menu_order":80,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/pages\/85"}],"collection":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/comments?post=85"}],"version-history":[{"count":5,"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/pages\/85\/revisions"}],"predecessor-version":[{"id":2524,"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/pages\/85\/revisions\/2524"}],"up":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/pages\/18"}],"wp:attachment":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/media?parent=85"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}