{"id":67,"date":"2018-01-08T10:14:45","date_gmt":"2018-01-08T10:14:45","guid":{"rendered":"http:\/\/confolab.sav.sk\/ovsb\/?page_id=67"},"modified":"2023-10-05T12:43:39","modified_gmt":"2023-10-05T12:43:39","slug":"priprava-vzoriek","status":"publish","type":"page","link":"https:\/\/confolab.sav.sk\/ovsb\/laboratoria\/priprava-vzoriek\/","title":{"rendered":"Pr\u00edprava vzoriek"},"content":{"rendered":"<h2><span class=\"H3\">Ultramikrot\u00f3m RMC PowerTome (Boeckeler Instruments), diamantov\u00fd n\u00f4\u017e (Diatome AG, \u0160vaj\u010diarsko), vibrat\u00f3m 7000smz (Campden Instruments), perf\u00fazne zariadenia<\/span>\u00a0\u00a0\u00a0\u00a0\u00a0<button>Nasp\u00e4\u0165<\/button><\/h2>\n<p><script>\t \t \nfunction goBack() { window.history.go(-1); }\t \t \n<\/script><\/p>\n<h3>Kontakt: RNDr. Marta Novotov\u00e1, CSc., RNDr. Ivan Zahradn\u00edk, CSc.<\/h3>\n<p>Na elektr\u00f3novo-mikroskopick\u00e9 sledovanie ultra\u0161trukt\u00fary sa zo vzorky tkaniva po zaliat\u00ed do polym\u00e9rnej \u017eivice <b>Durcupan <\/b> pripravuj\u00fa ultratenk\u00e9 rezy (58 \u2013 60 nm) na <b>ultramikrot\u00f3me<\/b>. Na z\u00edskanie ultratenk\u00fdch rezov je vyu\u017e\u00edvan\u00fd <b>diamantov\u00fd n\u00f4\u017e<\/b>. Ultratenk\u00e9 rezy nan\u00e1\u0161an\u00e9 na meden\u00e9 sie\u0165ky pokryt\u00e9 formvarovou blanou sl\u00fa\u017eia na \u0161t\u00fadium vzoriek pod elektr\u00f3nov\u00fdm mikroskopom.<\/p>\n<p><b> <img decoding=\"async\" loading=\"lazy\" class=\"wp-image-597 aligncenter\" src=\"https:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/mikrotom-768x252.png\" alt=\"mikrotom\" width=\"708\" height=\"231\" \/><\/b><\/p>\n<p>Perf\u00fazna aparat\u00fara na <b>chemick\u00fa fix\u00e1ciu izolovan\u00e9ho pracuj\u00faceho srdca<\/b> experiment\u00e1lnych zvierat (potkan, my\u0161) pozost\u00e1va z 3 perf\u00faznych kol\u00f3n. Kol\u00f3na \u010d.1 je naplnen\u00e1 fyziologick\u00fdm roztokom, ktor\u00fd sl\u00fa\u017ei na odstr\u00e1nenie krvi z pracuj\u00faceho srdca, kol\u00f3na \u010d. 2 obsahuj\u00faca fyziologick\u00fd roztok bez v\u00e1pnika sa pou\u017e\u00edva na vyvolanie relaxovan\u00e9ho stavu myokardu a kol\u00f3na \u010d.3 naplnen\u00e1 fixa\u010dn\u00fdm roztokom zabezpe\u010duje predfix\u00e1ciu cel\u00e9ho myokardu.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"alignright wp-image-603\" src=\"https:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/perfuzia-300x210.png\" alt=\"perfuzia\"  width=\"184\" height=\"129\" \/>Perf\u00fazna aparat\u00fara na<b> izol\u00e1ciu kardiomyocytov <\/b>z \u013eavej komory srdca pozost\u00e1va z 3 perf\u00faznych kol\u00f3n. Kol\u00f3na \u010d.1 je naplnen\u00e1 fyziologick\u00fdm roztokom, ktor\u00fd sl\u00fa\u017ei na odstr\u00e1nenie krvi z pracuj\u00faceho srdca, kol\u00f3na \u010d. 2 obsahuj\u00faca fyziologick\u00fd roztok bez v\u00e1pnika sa pou\u017e\u00edva na vyvolanie relaxovan\u00e9ho stavu myokardu a kol\u00f3na \u010d.3 naplnen\u00e1 enz\u00fdmov\u00fdm roztokom sl\u00fa\u017ei na oddelenie jednotliv\u00fdch buniek.<\/p>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td width=\"72%\">Na sledovanie \u0161trukt\u00fary svalov pracujeme s k\u00faskom cel\u00e9ho tkaniva, ktor\u00fd mechanicky vypreparujeme. Tenk\u00e9 rezy \u017eiv\u00e9ho tkaniva (10 \u2013 450 \u00b5m) pripravujeme na vibrat\u00f3me pomocou no\u017ea s \u010depe\u013eou z nehrdzavej\u00facej ocele alebo s keramickou \u010depe\u013eou z ultratvrd\u00e9ho zirk\u00f3nu.<\/td>\n<td width=\"5%\"><\/td>\n<td><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-846 \" src=\"https:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/vibratome-300x199-300x199.jpg\" alt=\"vibratome\" width=\"141\" height=\"94\" \/><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p>Ultramikrot\u00f3m RMC PowerTome (Boeckeler Instruments), diamantov\u00fd n\u00f4\u017e (Diatome AG, \u0160vaj\u010diarsko), vibrat\u00f3m 7000smz (Campden Instruments), perf\u00fazne zariadenia\u00a0\u00a0\u00a0\u00a0\u00a0Nasp\u00e4\u0165 Kontakt: RNDr. Marta Novotov\u00e1, CSc., RNDr. Ivan Zahradn\u00edk, CSc&#8230;.<span class=\"excerpt_more\"><a href=\"https:\/\/confolab.sav.sk\/ovsb\/laboratoria\/priprava-vzoriek\/\">\u010d\u00edta\u0165 \u010falej &raquo;<\/a><\/span><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":18,"menu_order":50,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/pages\/67"}],"collection":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/comments?post=67"}],"version-history":[{"count":15,"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/pages\/67\/revisions"}],"predecessor-version":[{"id":2522,"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/pages\/67\/revisions\/2522"}],"up":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/pages\/18"}],"wp:attachment":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/media?parent=67"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}