{"id":430,"date":"2018-01-10T11:57:19","date_gmt":"2018-01-10T11:57:19","guid":{"rendered":"http:\/\/confolab.sav.sk\/ovsb\/?page_id=430"},"modified":"2023-10-06T13:31:01","modified_gmt":"2023-10-06T13:31:01","slug":"vega-2-0190-10","status":"publish","type":"page","link":"https:\/\/confolab.sav.sk\/ovsb\/projekty\/ukoncene-projekty\/vega-2-0190-10\/","title":{"rendered":"VEGA 2\/0190\/10"},"content":{"rendered":"<p><script type=\"text\/javascript\">\n<!--\nfunction launch(newURL, newName, newFeatures, orgName) {\n  var remote = open(newURL, newName, newFeatures);\n  if (remote.opener == null)\n    remote.opener = window;\n  remote.opener.name = orgName;\n  return remote;\n}\n\nfunction launchRemote(name) {\n  var address =  name;\n  myRemote = launch(address ,\n                    \"Okno\",\n                    \"height=500, width=850, left=50, top=50, alwaysLowered=0, alwaysRaised=0, channelmode=0, dependent=0, directories=0, fullscreen=0, hotkeys=1, location=0, menubar=0, resizable=0, scrollbars=0, status=0, titlebar=1, toolbar=0, z-lock=0\",\n                    \"myWindow\");\n}\n\/\/ -->>\n<\/script><\/p>\n<h2>Mechanizmy regul\u00e1cie diastolickej aktivity ryanod\u00ednov\u00e9ho receptora &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<button onclick=\"goBack()\">Nasp\u00e4\u0165<\/button> <\/h2>\n<p><script>\t \t \nfunction goBack() { window.history.go(-1); }\t \t \n<\/script><\/p>\n<table border=\"0\" width=\"100%\" cellspacing=\"1\" cellpadding=\"2\">\n<tbody>\n<tr>\n<td>\n<h3>Ved\u00faci projektu: <a href=\"https:\/\/confolab.sav.sk\/ovsb\/pracovnici\/vyskumnici\/ing-alexandra-zahradnikova-drsc\/\">Alexandra Zahradn\u00edkov\u00e1<\/a><\/h3>\n<h3>Trvanie: janu\u00e1r 2010 &#8211; december 2012<br \/>\nKoordinuj\u00faca organiz\u00e1cia: \u00dastav molekul\u00e1rnej fyziol\u00f3gie a genetiky SAV, Bratislava<\/h3>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<h3><span class=\"H3\">Anot\u00e1cia:<\/span><\/h3>\n<p>Poruchy diastolick\u00fdch funkci\u00ed myokardu, ku ktor\u00fdm doch\u00e1dza napr. pri zlyhan\u00ed srdca alebo v d\u00f4sledku mut\u00e1cii v\u00e1pnikov\u00fdch kan\u00e1lov RyR, m\u00f4\u017eu vyvola\u0165 jednak zn\u00ed\u017eenie obsahu v\u00e1pnika vo vn\u00fatrobunkov\u00fdch z\u00e1sobn\u00edkoch, ale aj nestimulovan\u00e9 uvo\u013enenie v\u00e1pnika po\u010das diastoly. Nestimulovan\u00e9 uvo\u013enenie v\u00e1pnika generuje pred\u010dasn\u00e9 ak\u010dn\u00e9 potenci\u00e1ly prostredn\u00edctvom aktiv\u00e1cie Na-Ca v\u00fdmenn\u00edka. Diastolick\u00e1 regul\u00e1cia obsahu v\u00e1pnika vo vn\u00fatrobunkov\u00fdch z\u00e1sobn\u00edkoch prostredn\u00edctvom ryanod\u00ednov\u00e9ho receptora (RyR) sa d\u00e1 pozorova\u0165 vo forme tzv.<br \/>\nv\u00e1pnikov\u00fdch z\u00e1bleskov pomocou konfok\u00e1lnej mikroskopie. Regul\u00e1cia aktivity RyR po\u010das diastoly, t.j. pri n\u00edzkej hladine cytoplazmatick\u00e9ho v\u00e1pnika nie je doposia\u013e objasnen\u00e1. V tomto projekte sa zameriavame na \u0161t\u00fadium modul\u00e1cie aktivity RyR prirodzen\u00fdmi regul\u00e1tormi ako ATP, Mg2+ a lumin\u00e1lnym v\u00e1pnikom na \u00farovni jednotliv\u00fdch RyR kan\u00e1lov a izolovan\u00fdch srdcov\u00fdch svalov\u00fdch buniek. V\u00fdstupom projektu bude pochopenie riadiacich mechanizmov RyR po\u010das diastoly a identifik\u00e1cia ich por\u00fach pri srdcov\u00fdch ochoreniach.<\/td>\n<\/tr>\n<tr>\n<td>\n<h3><span class=\"H3\">K\u013e\u00fa\u010dov\u00e9 slov\u00e1:<\/span><\/h3>\n<p>v\u00e1pnikov\u00e1 homeost\u00e1za, ventrikul\u00e1rny myocyt, ryanod\u00ednov\u00fd receptor, plan\u00e1rne lipidick\u00e9 dvojvrstvy, patch clamp, konfok\u00e1lna mikroskopia, matematick\u00e9 modelovanie<\/td>\n<\/tr>\n<tr>\n<td>\n<h3><span class=\"H3\">Ciele:<\/span><\/h3>\n<p>Celkov\u00fdm cie\u013eom projektu je charakterizova\u0165 mechanizmy regul\u00e1cie aktivity RyR po\u010das diastoly a d\u00f4sledky por\u00fach t\u00fdchto mechanizmov pri srdcov\u00fdch ochoreniach. Na dosiahnutie tohto cie\u013ea sme si vyt\u00fd\u010dili nasledovn\u00e9 \u0161pecifick\u00e9 ciele:<\/p>\n<ul>\n<li> Z\u00edska\u0165 kvantitat\u00edvne \u00fadaje o regul\u00e1cii aktivity ryanod\u00ednov\u00e9ho receptora pri n\u00edzkych koncentr\u00e1ci\u00e1ch v\u00e1pnika, zodpovedaj\u00facim podmienkam in situ po\u010das diastoly, prirodzen\u00fdmi regul\u00e1tormi <\/li>\n<li> Z\u00edska\u0165 kvantitat\u00edvne \u00fadaje o \u00fa\u010dinku prirodzen\u00fdch regul\u00e1torov RyR na obsah v\u00e1pnika v sarkoplazmatickom retikule (SR) a na uvo\u013e\u0148ovanie v\u00e1pnika zo SR. <\/li>\n<li> S pomocou z\u00edskan\u00fdch \u00fadajov ur\u010di\u0165 d\u00f4sledky por\u00fach interakcie RyR s prirodzen\u00fdmi regul\u00e1tormi na diastolick\u00e9 uvo\u013e\u0148ovanie v\u00e1pnika zo SR a n\u00e1sledne na v\u00e1pnikov\u00fa homeost\u00e1zu srdcov\u00e9ho myocytu. <\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<h3><span class=\"H3\">Publik\u00e1cie:<\/span><\/h3>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<table class=\"list\" border=\"0\" width=\"100%\" cellspacing=\"0\" cellpadding=\"4\">\n<tbody>\n<tr>\n<td class=\"small\"><a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22934071\")'><img decoding=\"async\" class=\"alignnone wp-image-1111\" src=\"https:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/FrontiersPhysiol.jpg\" alt=\"FrontiersPhysiol\" width=\"75\" \/><\/a><\/td>\n<td class=\"two\"><strong>Zahradnikova A<\/strong>, <strong>Zahradnik I<\/strong> (2012). <a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22934071\")'>Construction of calcium release sites in cardiac myocytes<\/a>. <i>Front Physiol<\/i> 3: 322.<\/td>\n<\/tr>\n<tr>\n<td class=\"small\"><a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22890710\")'><img decoding=\"async\" class=\"alignnone wp-image-1113\" src=\"https:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/JPhysiol3.jpg\" alt=\"JPhysiol3\" width=\"75\" \/><\/a><\/td>\n<td class=\"two\"><strong>Janicek R<\/strong>, <strong>Zahradnikova-Jr A<\/strong>, <strong>Polakova E<\/strong>, <strong>Pavelkova J<\/strong>, <strong>Zahradnik I<\/strong>, <strong>Zahradnikova A<\/strong> (2012): <a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22890710\")'>Calcium spike variability in cardiac myocytes results from activation of small cohorts of RYR2 channels.<\/a> <i>J Physiol<\/i> 590: 5091-5106.<\/td>\n<\/tr>\n<tr>\n<td class=\"small\"><a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22851674\")'><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-1114\" src=\"https:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/JGP-125x150.jpg\" alt=\"JGP\" width=\"75\" height=\"98\" \/><\/a><\/td>\n<td class=\"two\"><strong>Tencerova B<\/strong>, <strong>Zahradnikova A<\/strong>, <strong>Gaburjakova J,<\/strong> <strong>Gaburjakova M<\/strong> (2012): <a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22851674\")'>Luminal Ca2+ controls activation of the cardiac ryanodine receptor by ATP<\/a>. <i>J Gen Physiol<\/i> 140: 93-108.<\/td>\n<\/tr>\n<tr>\n<td class=\"small\"><a href='javascript:launchRemote(\"https:\/\/cdn.intechopen.com\/pdfs-wm\/22502.pdf\")'><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"https:\/\/cdn.intechopen.com\/books\/images\/1326.jpg\" width=\"75\" height=\"104\" \/><\/a><\/td>\n<td class=\"two\">Bauerova-Hlinkova V,Bauer J, Hostinova E, Gasperik J, Beck K, Borko L, <strong> Faltinova A<\/strong>, <strong>Zahradnikova A<\/strong>, Sevcik J (2011): <a href='javascript:launchRemote(\"https:\/\/cdn.intechopen.com\/pdfs-wm\/22502.pdf\")'>Bioinformatics Domain Structure Prediction and Homology Modeling of Human Ryanodine Receptor 2.<\/a> Editor Mahmood A. Mahdavi. In Bioinformatics-Trends and Methodologies. &#8211; Rijeka, p. 325-352. ISBN 978-953-307-282-1.<\/td>\n<\/tr>\n<tr>\n<td class=\"small\"><a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/20045464?dopt=Citation\")'><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"https:\/\/ars.els-cdn.com\/content\/image\/1-s2.0-S1046592810X00043-cov150h.gif\" width=\"75\" height=\"104\" \/><\/a><\/td>\n<td class=\"two\">Bauerova-Hlinkova V, Hostinova E, Gasperik J, Beck K, Borko L, Lai FA, <strong> Zahradnikova A<\/strong>, Sevcik J (2010): <a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/20045464?dopt=Citation\")'>Bioinformatic mapping and production of recombinant N-terminal domains of human cardiac ryanodine receptor 2.<\/a>. <i>Protein Expr. Purif<\/i> 71:33-41.<\/td>\n<\/tr>\n<tr>\n<td class=\"small\"><a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/20974774?dopt=Citation\")'><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-1114\" src=\"https:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/JGP-125x150.jpg\" alt=\"JGP\" width=\"75\" height=\"98\" \/><\/a><\/td>\n<td class=\"two\"><strong> Zahradnikova A<\/strong>, <strong> Gaburjakova M<\/strong> Bridge JHB, <strong> Zahradnik I<\/strong> (2010): <a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/20974774?dopt=Citation\")'>Challenging quantal calcium signaling in cardiac myocytes.<\/a>. <i>J Gen Physiol<\/i> 136:581-583.<\/td>\n<\/tr>\n<tr>\n<td class=\"small\"><a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/20548054\")'><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-1114\" src=\"https:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/JGP-125x150.jpg\" alt=\"JGP\" width=\"75\" height=\"98\" \/><\/a><\/td>\n<td class=\"two\"><strong>Zahradnikova A<\/strong>, <strong>Valent I<\/strong>, <strong>Zahradnik I<\/strong> (2010): <a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/20548054\")'>Frequency and release flux of calcium sparks in rat cardiac myocytes: a relation to RYR gating.<\/a> <i> J Gen Physiol<\/i> 136: 101-116.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p>Mechanizmy regul\u00e1cie diastolickej aktivity ryanod\u00ednov\u00e9ho receptora &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Nasp\u00e4\u0165 Ved\u00faci projektu: Alexandra Zahradn\u00edkov\u00e1 Trvanie: janu\u00e1r 2010 &#8211; december 2012 Koordinuj\u00faca organiz\u00e1cia: \u00dastav molekul\u00e1rnej fyziol\u00f3gie a genetiky SAV,&#8230;<span class=\"excerpt_more\"><a href=\"https:\/\/confolab.sav.sk\/ovsb\/projekty\/ukoncene-projekty\/vega-2-0190-10\/\">\u010d\u00edta\u0165 \u010falej &raquo;<\/a><\/span><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":117,"menu_order":190,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/pages\/430"}],"collection":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/comments?post=430"}],"version-history":[{"count":5,"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/pages\/430\/revisions"}],"predecessor-version":[{"id":2584,"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/pages\/430\/revisions\/2584"}],"up":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/pages\/117"}],"wp:attachment":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/media?parent=430"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}