V\u00fdskum na OBK je zameran\u00fd na subcelul\u00e1rne mechanizmy excit\u00e1cie a kontrakcie srdcov\u00fdch svalov\u00fdch buniek \u2013 ve\u013ek\u00e9 t\u00e9my kardiol\u00f3gie, fyziol\u00f3gie a biofyziky. Na\u0161\u00edm cie \u013eom je prispie\u0165 p\u00f4vodn\u00fdmi my\u0161lienkami k objasneniu tejto problematiky, by\u0165 kompetit\u00edvnou s\u00fa\u010das\u0165ou medzin\u00e1rodnej vedeckej komunity a publikova\u0165 v popredn\u00fdch medzin\u00e1rodn\u00fdch \u010dasopisoch na\u0161ej vednej oblasti. Zameriavame sa na molekul\u00e1rne a \u0161trukt\u00farne mechanizmy syst\u00e9mu v\u00e1pnikovej signaliz\u00e1cie, ktor\u00e9 participuj\u00fa na excitabilite, kontraktilite a energetike srdcov\u00fdch myocytov. Na\u0161e projekty s\u00fa zalo\u017een\u00e9 na prepojen\u00ed najmodernej\u0161\u00edch experiment\u00e1lnych met\u00f3d merania aktivity membr\u00e1nov\u00fdch i\u00f3nov\u00fdch kan\u00e1lov, vn\u00fatrobunkovej aktivity v\u00e1pnika a dynamiky membr\u00e1nov\u00fdch \u0161trukt\u00far bunky s teoretick\u00fdmi met\u00f3dami, ktor\u00e9 zah\u0155\u0148aj\u00fa ich anal\u00fdzu a matematick\u00e9 modelovanie.<\/p>\n
Pou\u017e\u00edvame nasledovn\u00e9 experiment\u00e1lne techniky: izol\u00e1ciu funk\u010dn\u00fdch srdcov\u00fdch svalov\u00fdch buniek, mikroelektr\u00f3dov\u00fa met\u00f3du \u201cpatch clamp\u201d v kombin\u00e1cii s technikami konfok\u00e1lnej mikroskopie, rekon\u0161tit\u00faciu i\u00f3nov\u00fdch kan\u00e1lov v plan\u00e1rnych lipidov\u00fdch membr\u00e1nach, elektr\u00f3nov\u00fa mikroskopiu a anal\u00fdzu obrazu. Pre mnoh\u00e9 z t\u00fdchto met\u00f3d vytv\u00e1rame nov\u00fd softv\u00e9r na zber a anal\u00fdzu d\u00e1t. Vyvinuli sme unik\u00e1tne in silico modely otv\u00e1rania RYR2 kan\u00e1lov, tvorby v\u00e1pnikov\u00fdch sign\u00e1lov v bunke, ako aj 3D \u0161trukt\u00fary prie\u010dne pruhovan\u00fdch svalov\u00fdch buniek. V\u0161etky s\u00fa zalo\u017een\u00e9 na na\u0161ich v\u00fdsledkoch a na najnov\u0161\u00edch svetov\u00fdch poznatkoch. Tieto modely uplat\u0148ujeme pri formulovan\u00ed a verifikovan\u00ed pracovn\u00fdch hypot\u00e9z, pri interpret\u00e1cii experiment\u00e1lnych v\u00fdsledkov a pri ich komunikovan\u00ed \u0161ir\u0161ej odbornej verejnosti.<\/p>\n","protected":false},"excerpt":{"rendered":"
V\u00fdskum na OBK je zameran\u00fd na subcelul\u00e1rne mechanizmy excit\u00e1cie a kontrakcie srdcov\u00fdch svalov\u00fdch buniek \u2013 ve\u013ek\u00e9 t\u00e9my kardiol\u00f3gie, fyziol\u00f3gie a biofyziky. Na\u0161\u00edm cie \u013eom je…\u010d\u00edta\u0165 \u010falej »<\/a><\/span><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":16,"menu_order":10,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/pages\/33"}],"collection":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/comments?post=33"}],"version-history":[{"count":4,"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/pages\/33\/revisions"}],"predecessor-version":[{"id":746,"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/pages\/33\/revisions\/746"}],"up":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/pages\/16"}],"wp:attachment":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/wp-json\/wp\/v2\/media?parent=33"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}