{"id":389,"date":"2018-01-10T13:36:35","date_gmt":"2018-01-10T12:36:35","guid":{"rendered":"http:\/\/confolab.sav.sk\/ovsb\/en\/?page_id=389"},"modified":"2024-02-16T13:40:50","modified_gmt":"2024-02-16T12:40:50","slug":"vega-2-0197-11","status":"publish","type":"page","link":"https:\/\/confolab.sav.sk\/ovsb\/en\/projects\/completed-projects\/vega-2-0197-11\/","title":{"rendered":"VEGA 2\/0197\/11"},"content":{"rendered":"<p><script type=\"text\/javascript\">\n<!--\nfunction launch(newURL, newName, newFeatures, orgName) {\n  var remote = open(newURL, newName, newFeatures);\n  if (remote.opener == null)\n    remote.opener = window;\n  remote.opener.name = orgName;\n  return remote;\n}\n\nfunction launchRemote(name) {\n  var address =  name;\n  myRemote = launch(address ,\n                    \"Okno\",\n                    \"height=500, width=850, left=50, top=50, alwaysLowered=0, alwaysRaised=0, channelmode=0, dependent=0, directories=0, fullscreen=0, hotkeys=1, location=0, menubar=0, resizable=0, scrollbars=0, status=0, titlebar=1, toolbar=0, z-lock=0\",\n                    \"myWindow\");\n}\n\/\/ -->>\n<\/script><\/p>\n<h2>Mechanisms of communication among ion channels of cardiomyocyte dyad&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<button onclick=\"goBack()\">Back<\/button> <\/h2>\n<p><script>\t \t \nfunction goBack() { window.history.go(-1); }\t \t \n<\/script><\/p>\n<table border=\"0\" width=\"100%\" cellspacing=\"1\" cellpadding=\"2\">\n<tbody>\n<tr>\n<td>\n<h3>Principal Investigator: <a href=\"http:\/\/www.umfg.sav.sk\/ovsb\/en\/people\/zahradnikova-jr-a\/\">Alexandra Zahradn\u00edkov\u00e1, jr.<\/a>, <a href=\"http:\/\/www.umfg.sav.sk\/ovsb\/en\/people\/researchers\/pavelkova-j\/\">Jana Pavelkov\u00e1<\/a><\/h3>\n<h3>Duration: January 2011 &#8211; December 2013<br \/>\nCoordinating Organization: Institute of Molecular Physiology and Genetics SAS, Bratislava<\/h3>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<h3><span class=\"H3\">Annotation:<\/span><\/h3>\n<p>Robustness of excitation-contraction coupling in cardiomyocytes is locally ensured by the presence of a large number of DHPR and RyR channels in the dyad. Significant redundancy of the system allows, if necessary, to recruit more channels and thus continuously respond to the increased contractions. This project aims to study the mechanism of interaction between DHPR and RyR channels, mediated by calcium ions, in the cardiomyocytes in situ. We will use a combination of two modern experimental techniques. Voltage clamp method allows us to track response of DHPR channels to voltage stimulus and measure the calcium current value that is a stimulus activating the RyR channels. The method of confocal microscopy enables us to trace the local release of calcium via RyR channels and thereby directly measure their activity in situ. This project will help us understand better the mechanisms regulating communication between the DHPR and RyR channels, and allow us to study the changes of these mechanisms occurring during.<\/td>\n<\/tr>\n<tr>\n<td>\n<h3><span class=\"H3\">Keywords:<\/span><\/h3>\n<p>Calcium signalling, excitation-contraction coupling, cardiomyocytes, calcium current, ryanodine receptors<\/td>\n<\/tr>\n<tr>\n<td>\n<h3><span class=\"H3\">Objectives:<\/span><\/h3>\n<p>The project aims to further understanding of mechanisms of interaction between DHPR and RyR channels, conveyed by calcium ions, in dyads of cardiomyocytes. The questions that we specifically adress are:<\/p>\n<ul>\n<li> In what manner does the stimulating calcium affect the rate and amplitude of the local calcium release? <\/li>\n<li> To what extents are the fast and slow inactivation of calcium current dependent on the rate and amplitude of local calcium release? <\/li>\n<li> Which intracellular regulators of RyR channel activity are mostly involved in the modulation of the local calcium release? <\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<h3><span class=\"H3\">Publications:<\/span><\/h3>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<table class=\"list\" border=\"0\" width=\"100%\" cellspacing=\"0\" cellpadding=\"4\">\n<tbody>\n<tr>\n<td class=\"small\"><a href='javascript:launchRemote(\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/23741324\")'><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-1110\" src=\"http:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/PLoSOne.jpg\" alt=\"PLoSOne\" width=\"75\" height=\"54\" \/><\/a><\/td>\n<td class=\"two\"><strong>Janicek R<\/strong>, <strong>Hotka M<\/strong>, <strong>Zahradnikova-Jr A<\/strong>, <strong>Zahradnikova A<\/strong>, <strong>Zahradnik I<\/strong> (2013): <a href='javascript:launchRemote(\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/23741324\")'>Quantitative analysis of calcium spikes in noisy fluorescent background<\/a>. <i>PLoS One<\/i> 8: e64394.<\/td>\n<\/tr>\n<tr>\n<td class=\"small\"><a href='javascript:launchRemote(\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22934071\")'><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-1111\" src=\"http:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/FrontiersPhysiol-150x51.jpg\" alt=\"FrontiersPhysiol\" width=\"75\" height=\"17\" \/><\/a><\/td>\n<td class=\"two\"><strong>Zahradnikova A<\/strong>, <strong>Zahradnik I<\/strong> (2012). <a href='javascript:launchRemote(\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22934071\")'>Construction of calcium release sites in cardiac myocytes<\/a>. <i>Front Physiol<\/i> 3: 322.<\/td>\n<\/tr>\n<tr>\n<td class=\"small\"><a href='javascript:launchRemote(\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22890710\")'><img decoding=\"async\" class=\"alignnone wp-image-1113\" src=\"http:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/JPhysiol3.jpg\" alt=\"JPhysiol3\" width=\"75\" \/><\/a><\/td>\n<td class=\"two\"><strong>Janicek R<\/strong>, <strong>Zahradnikova-Jr A<\/strong>, <strong>Polakova E<\/strong>, <strong>Pavelkova J<\/strong>, <strong>Zahradnik I<\/strong>, <strong>Zahradnikova A<\/strong> (2012): <a href='javascript:launchRemote(\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22890710\")'>Calcium spike variability in cardiac myocytes results from activation of small cohorts of RYR2 channels.<\/a> <i>J Physiol<\/i> 590: 5091-5106.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p>Mechanisms of communication among ion channels of cardiomyocyte dyad&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Back Principal Investigator: Alexandra Zahradn\u00edkov\u00e1, jr., Jana Pavelkov\u00e1 Duration: January 2011 &#8211; December 2013 Coordinating Organization: Institute&#8230;<span class=\"excerpt_more\"><a href=\"https:\/\/confolab.sav.sk\/ovsb\/en\/projects\/completed-projects\/vega-2-0197-11\/\">\u010d\u00edta\u0165 \u010falej &raquo;<\/a><\/span><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":199,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages\/389"}],"collection":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/comments?post=389"}],"version-history":[{"count":4,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages\/389\/revisions"}],"predecessor-version":[{"id":1618,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages\/389\/revisions\/1618"}],"up":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages\/199"}],"wp:attachment":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/media?parent=389"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}