{"id":379,"date":"2018-01-10T10:09:20","date_gmt":"2018-01-10T09:09:20","guid":{"rendered":"http:\/\/confolab.sav.sk\/ovsb\/en\/?page_id=379"},"modified":"2024-02-16T13:37:20","modified_gmt":"2024-02-16T12:37:20","slug":"vega-2-0148-14","status":"publish","type":"page","link":"https:\/\/confolab.sav.sk\/ovsb\/en\/projects\/completed-projects\/vega-2-0148-14\/","title":{"rendered":"VEGA 2\/0148\/14"},"content":{"rendered":"<p><script type=\"text\/javascript\">\n<!--\nfunction launch(newURL, newName, newFeatures, orgName) {\n  var remote = open(newURL, newName, newFeatures);\n  if (remote.opener == null)\n    remote.opener = window;\n  remote.opener.name = orgName;\n  return remote;\n}\n\nfunction launchRemote(name) {\n  var address =  name;\n  myRemote = launch(address ,\n                    \"Okno\",\n                    \"height=500, width=850, left=50, top=50, alwaysLowered=0, alwaysRaised=0, channelmode=0, dependent=0, directories=0, fullscreen=0, hotkeys=1, location=0, menubar=0, resizable=0, scrollbars=0, status=0, titlebar=1, toolbar=0, z-lock=0\",\n                    \"myWindow\");\n}\n\/\/ -->>\n<\/script><\/p>\n<h2>Structure-function relationships of the ryanodine receptor&nbsp; &nbsp; &nbsp; &nbsp;<button onclick=\"goBack()\">Back<\/button> <\/h2>\n<p><script>\t \t \nfunction goBack() { window.history.go(-1); }\t \t \n<\/script><\/p>\n<table border=\"0\" width=\"100%\" cellspacing=\"1\" cellpadding=\"2\">\n<tbody>\n<tr>\n<td>\n<h3>Principal Investigator: <a href=\"http:\/\/www.umfg.sav.sk\/ovsb\/en\/people\/researchers\/zahradnikova-a\/\">Alexandra Zahradn\u00edkov\u00e1<\/a><\/h3>\n<h3>Duration: January 2014 &#8211; December 2016<br \/>\nCoordinating Organization: Institute of Molecular Physiology and Genetics SAS, Bratislava<\/h3>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<h3><span class=\"H3\">Annotation:<\/span><\/h3>\n<p>Correct functioning of ryanodine receptors (RyRs) is crucial for proper myocardial function. Ryanodine receptors maintain calcium homeostasis by regulation of diastolic calcium leak, and ensure proper extent of contraction by regulation of calcium-induced calcium release during the systole. In civilization diseases of the heart and in some inherited arrhythmias, RyR function is disturbed as a result changed phosphorylation, redox state, or RyR mutations, which leads to decreased contraction and increase of diastolic calcium release. The structure-function relationships of RyR from the viewpoint of regulation of its activity are not well understood. In this project we will focus on deciphering modulation of RyR activity at the level of single channels, dyadic channel complexes, and isolated cardiac myocytes. The results will enable deeper understanding of the mechanisms that govern RyR activity during systole and diastole.<\/td>\n<\/tr>\n<tr>\n<td>\n<h3><span class=\"H3\">Keywords:<\/span><\/h3>\n<p>calcium homeostasis, calcium signaling, ryanodine receptor, patch clamp, confocal microscopy, planar lipid bilayers, mathematical modelling, bioinformatics<\/td>\n<\/tr>\n<tr>\n<td>\n<h3><span class=\"H3\">Objectives:<\/span><\/h3>\n<p>The overall objective of the project is to characterize the mechanisms of regulation of the activity of the ryanodine receptor during systole as well as diastole and the consequences of their defects in cardiac diseases. The specific aims to reach this goal are:<\/p>\n<ul>\n<li>To obtain quantitative data on regulation of RyR activity at rest and in response to threshold and maximal stimuli in vitro a in situ.<\/li>\n<li>Using modern methods of bioinformatics, to build a molecular model of the 3D structure of the RyR channel.<\/li>\n<li>By interpreting the above results, to estimate the consequences of defects of interaction between RyR and its ligands on the diastolic and systolic activity of RyR and consequentially on calcium homeostasis of the cardiac myocyte.<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<h3><span class=\"H3\">Publications:<\/span><\/h3>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<table class=\"list\" border=\"0\" width=\"100%\" cellspacing=\"0\" cellpadding=\"4\">\n<tbody>\n<tr>\n<td class=\"small\"><a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28713282\")'><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-1111\" src=\"http:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/FrontiersPhysiol-150x51.jpg\" alt=\"FrontiersPhysiol\" width=\"75\" height=\"17\" \/><\/a><\/td>\n<td class=\"two\"><strong>Faltinova A<\/strong>, Tomaskova N, Antalik M, Sevcik J, <strong>Zahradnikova A<\/strong> (2017). <a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28713282\")'>The N-terminal region of the ryanodine receptor affects channel activation<\/a>. <i>Front Physiol<\/i> 8: 443.<\/td>\n<\/tr>\n<tr>\n<td class=\"small\" width=\"5%\"><a href='javascript:launchRemote(\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26009544\")'><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-1114\" src=\"http:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/JGP-125x150.jpg\" alt=\"JGP\" width=\"80\" height=\"104\" \/><\/a><\/td>\n<td class=\"two\">Petrovic P, <strong>Valent I<\/strong>, <strong>Cocherova E<\/strong>, <strong>Pavelkova J<\/strong>, <strong>Zahradnikova A<\/strong> (2015): <a href='javascript:launchRemote(\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26009544\")'>Ryanodine receptor gating controls generation of diastolic calcium waves in cardiac myocytes<\/a>. <i>J Gen Physiol<\/i> 145: 489-511.<\/td>\n<\/tr>\n<tr>\n<td class=\"small\"><a href='javascript:launchRemote(\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25372681\")'><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-1125\" src=\"http:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/ActaCryst-150x150.jpg\" alt=\"ActaCryst\" width=\"75\" height=\"97\" \/><\/a><\/td>\n<td class=\"two\">Borko L, Bauerova-Hlinkova V, Hostinova E, Gasparik J, Beck K, Lai FA, <strong>Zahradnikova A<\/strong>, Sevcik J (2014): <a href='javascript:launchRemote(\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25372681\")'>Structural insights into the human RyR2 N-terminal region involved in cardiac arrhythmia<\/a>s. <i>Acta Cryst<\/i> D70: 2897\u20132912.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p>Structure-function relationships of the ryanodine receptor&nbsp; &nbsp; &nbsp; &nbsp;Back Principal Investigator: Alexandra Zahradn\u00edkov\u00e1 Duration: January 2014 &#8211; December 2016 Coordinating Organization: Institute of Molecular Physiology&#8230;<span class=\"excerpt_more\"><a href=\"https:\/\/confolab.sav.sk\/ovsb\/en\/projects\/completed-projects\/vega-2-0148-14\/\">\u010d\u00edta\u0165 \u010falej &raquo;<\/a><\/span><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":199,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages\/379"}],"collection":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/comments?post=379"}],"version-history":[{"count":3,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages\/379\/revisions"}],"predecessor-version":[{"id":1615,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages\/379\/revisions\/1615"}],"up":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages\/199"}],"wp:attachment":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/media?parent=379"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}