{"id":312,"date":"2018-01-08T18:05:01","date_gmt":"2018-01-08T17:05:01","guid":{"rendered":"http:\/\/confolab.sav.sk\/ovsb\/en\/?page_id=312"},"modified":"2024-02-16T13:24:00","modified_gmt":"2024-02-16T12:24:00","slug":"vega-2-0143-17","status":"publish","type":"page","link":"https:\/\/confolab.sav.sk\/ovsb\/en\/projects\/completed-projects\/vega-2-0143-17\/","title":{"rendered":"VEGA 2\/0143\/17"},"content":{"rendered":"<p><script type=\"text\/javascript\">\n<!--\nfunction launch(newURL, newName, newFeatures, orgName) {\n  var remote = open(newURL, newName, newFeatures);\n  if (remote.opener == null)\n    remote.opener = window;\n  remote.opener.name = orgName;\n  return remote;\n}\n\nfunction launchRemote(name) {\n  var address =  name;\n  myRemote = launch(address ,\n                    \"Okno\",\n                    \"height=500, width=850, left=50, top=50, alwaysLowered=0, alwaysRaised=0, channelmode=0, dependent=0, directories=0, fullscreen=0, hotkeys=1, location=0, menubar=0, resizable=0, scrollbars=0, status=0, titlebar=1, toolbar=0, z-lock=0\",\n                    \"myWindow\");\n}\n\/\/ -->>\n<\/script><\/p>\n<h2>Diastolic function of the ryanodine receptor and generation of arrhythmogenic calcium waves&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<button onclick=\"goBack()\">Back<\/button> <\/h2>\n<p><script>\t \t \nfunction goBack() { window.history.go(-1); }\t \t \n<\/script><\/p>\n<table border=\"0\" width=\"100%\" cellspacing=\"1\" cellpadding=\"2\">\n<tbody>\n<tr>\n<td>\n<h3>Principal Investigator: <a href=\"http:\/\/confolab.sav.sk\/ovsb\/en\/people\/researchers\/ing-alexandra-zahradnikova-drsc\/\">Alexandra Zahradn\u00edkov\u00e1<\/a> (2017); <a href=\"http:\/\/confolab.sav.sk\/ovsb\/en\/people\/researchers\/rndr-alexandra-zahradnikova-jr-phd\/\">Alexandra Zahradn\u00edkov\u00e1 jr.<\/a> (2018-2019)<br \/>\n<\/h3>\n<h3>Duration: January 2017 &#8211; December 2019<br \/>\nCoordinating Organization: Institute of Molecular Physiology and Genetics SAS, Bratislava (2017); Institute of Experimental Endocrinology BRC SAS (2018-2019)<\/h3>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<h3><span class=\"H3\">Annotation:<\/span><\/h3>\n<p>In some acquired and genetic arrhythmias, anomalies in calcium release occur during the diastole, which may result in formation of calcium waves that initiate cardiac arrhythmias. Proper diastolic function of the system of calcium homeostasis involves regulation of diastolic calcium release by ryanodine receptors. Anomalous calcium release from the viewpoint of calcium wave formation is not sufficiently understood. We will concentrate on determination of the relationships between localization of dyads as the sites of calcium release and formation of calcium waves, and on their development during maturation and physiological hypertrophy of myocytes. The outcome of the project will be a better understanding of the factors governing calcium homeostasis in cardiac myocytes and their impairment leading to calcium waves.<\/td>\n<\/tr>\n<tr>\n<td>\n<h3><span class=\"H3\">Keywords:<\/span><\/h3>\n<p>Myocardium, myocyte, calcium homeostasis, calcium signalling, ryanodine receptor, super- resolution STED microscopy, mathematical modelling<\/td>\n<\/tr>\n<tr>\n<td>\n<h3><span class=\"H3\">Objectives:<\/span><\/h3>\n<p>The overall objective of the project is to characterize regulation of diastolic RyR activity during ontogenesis and physiological hypertrophy. To reach this goal we have formulated the following specific aims:<\/p>\n<ul>\n<li> To obtain quantitative data on formation of calcium waves during cardiomyocyte maturation and physiological hypertrophy and on possible pharmacological intervention <\/li>\n<li> To obtain quantitative data on localization and environment of dyads &#8211; calcium release sites &#8211; during maturation and physiological hypertrophy of cardiac myocytes <\/li>\n<li> To assess the relationship between localization and environment of dyads and the propensity of myocytes to generation of calcium waves. <\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<h3><span class=\"H3\">Publications:<\/span><\/h3>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<table class=\"list\" border=\"0\" width=\"100%\" cellspacing=\"0\" cellpadding=\"4\">\n<tbody>\n<tr>\n<td class=\"small\"><a href='javascript:launchRemote(\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33084603\/\")'><img decoding=\"async\" loading=\"lazy\" width=\"75\" height=\"104\" class=\"alignnone\" src=\"http:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/GPB-1.jpg\"><\/a><\/td>\n<td class=\"two\">Kurekova S, Plaas M, <strong>Cagalinec M<\/strong> (2020): <a href='javascript:launchRemote(\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33084603\/\")'>Short Communication: Lack of functional wolframin causes drop in  plasmalemmal sodium-calcium exchanger type  1 expression at  early stage in  rat model of  Wolfram syndrome<\/a>. <i>Gen Physiol Biophys<\/i> 39: 499\u2013503 doi: 10.4149\/gpb_2020017.<\/td>\n<\/tr>\n<tr>\n<td class=\"small\"><a href='javascript:launchRemote(\"https:\/\/www.biorxiv.org\/content\/10.1101\/2020.08.26.260968v1\")'><img decoding=\"async\" class=\"alignnone wp-image-1111\" src=\"http:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/bioRxiv_article.jpg\" alt=\"Prog Biophys Mol Biol\" width=\"75\" \/><\/a><\/td>\n<td class=\"two\"> <strong>Iaparov B<\/strong>, <strong>Zahradnik I<\/strong>, Moskvin AS, <strong>Zahradnikova A<\/strong> (2020): <a href='javascript:launchRemote(\"https:\/\/www.biorxiv.org\/content\/10.1101\/2020.08.26.260968v1\")'>Synergy of calcium release site determinants in control of calcium release events in cardiac myocytes<\/a>. <i>bioRXiv<\/i> :  doi: https:\/\/doi.org\/10.1101\/2020.08.26.260968.<\/td>\n<\/tr>\n<tr>\n<td class=\"small\"><a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/32416189\")'><img decoding=\"async\" class=\"alignnone wp-image-1111\" src=\"http:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/ProgBiophysMolBiol-e1590139461171.jpg\" alt=\"Prog Biophys Mol Biol\" width=\"75\" \/><\/a><\/td>\n<td class=\"two\"><strong>Zahradnikova A<\/strong>, <strong>Iaparov B<\/strong>, <strong>Zahradnik I<\/strong> (2020): <a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/32416189\")'>The problem of accuracy in single-channel open probability measurements<\/a>. <i>Prog Biophys Mol Biol<\/i> 157: 94-106 doi: 10.1016\/j.pbiomolbio.2020.05.002.<\/td>\n<\/tr>\n<tr>\n<td class=\"small\"><a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/32415205\")'><img decoding=\"async\" width=\"75\" class=\"alignnone wp-image-1110\" alt=\"SciRep\" src=\"http:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/SciRep-1-300x111.jpg\"><\/a><\/td>\n<td class=\"two\"><strong>Novotova M<\/strong>, <strong>Zahradnikova A Jr<\/strong>, Nichtova Z, Kovac R, Kralova E, Stankovicova T, <strong>Zahradnikova A<\/strong>, <strong>Zahradnik I<\/strong> (2020): <a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/32415205\")'>Structural variability of dyads relates to calcium release in rat ventricular myocytes<\/a>. <i>Sci Rep<\/i> 10: 8076 doi: 10.1038\/s41598-020-64840-5.<\/td>\n<\/tr>\n<tr>\n<td class=\"small\"><a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/31236612\")'><img decoding=\"async\" class=\"alignnone wp-image-1111\" src=\"http:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/EurBiophysJ.jpg\" alt=\"Eur Biophys J\" width=\"75\" \/><\/a><\/td>\n<td class=\"two\">Iaparov B, Moskvin AS, <strong>Zahradnik I<\/strong>, <strong>Zahradnikova A<\/strong> (2019): <a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/31236612\")'>Stochastic and deterministic approaches to modelling calcium release in cardiac myocytes at different spatial arrangements of ryanodine receptors<\/a>. <i>Eur Biophys J<\/i> 48: 579\u2013584 doi: 10.1007\/s00249-019-01378-z.<\/td>\n<\/tr>\n<tr>\n<td class=\"small\"><a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/31145728\")'><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-1110\" src=\"http:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/PLoSOne.jpg\" alt=\"PLoSOne\" width=\"75\" height=\"54\" \/><\/a><\/td>\n<td class=\"two\">Skrabanek P, <strong>Zahradnikova A Jr<\/strong> (2019): <a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/31145728\")'>Automatic assessment of the cardiomyocyte development stages from confocal microscopy images using deep convolutional networks<\/a>. <i>PLoS One<\/i> 14: e0216720, doi: 10.1371\/journal.pone.0216720.<\/td>\n<\/tr>\n<tr>\n<td class=\"small\"><a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/30930784\")'><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-1111\" src=\"http:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/FrontiersPhysiol-150x51.jpg\" alt=\"FrontiersPhysiol\" width=\"75\" height=\"17\" \/><\/a><\/td>\n<td class=\"two\"><strong>Cagalinec M<\/strong>, <strong>Zahradnikova A<\/strong>, <strong>Zahradnikova A Jr<\/strong>, Kovacova D, Paulis L, <strong>Kurekova S<\/strong>, <strong>Hotka M<\/strong>, <strong>Pavelkova J<\/strong>, Plaas M, <strong>Novotova M<\/strong>, <strong>Zahradnik I<\/strong> (2019). <a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/30930784\")'>Calcium signaling and contractility in cardiac myocyte of wolframin deficient rats<\/a>. <i>Front Physiol<\/i> 10: 172, doi: 10.3389\/fphys.2019.00172.<\/td>\n<\/tr>\n<tr>\n<td class=\"small\"><a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28713282\")'><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-1111\" src=\"http:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/FrontiersPhysiol-150x51.jpg\" alt=\"FrontiersPhysiol\" width=\"75\" height=\"17\" \/><\/a><\/td>\n<td class=\"two\"><strong>Faltinova A<\/strong>, Tomaskova N, Antalik M, Sevcik J, <strong>Zahradnikova A<\/strong> (2017). <a href='javascript:launchRemote(\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28713282\")'>The N-terminal region of the ryanodine receptor affects channel activation<\/a>. <i>Front Physiol<\/i> 8: 443.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p>Diastolic function of the ryanodine receptor and generation of arrhythmogenic calcium waves&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Back Principal Investigator: Alexandra Zahradn\u00edkov\u00e1 (2017); Alexandra Zahradn\u00edkov\u00e1 jr. (2018-2019) Duration: January 2017 &#8211;&#8230;<span class=\"excerpt_more\"><a href=\"https:\/\/confolab.sav.sk\/ovsb\/en\/projects\/completed-projects\/vega-2-0143-17\/\">\u010d\u00edta\u0165 \u010falej &raquo;<\/a><\/span><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":199,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages\/312"}],"collection":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/comments?post=312"}],"version-history":[{"count":14,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages\/312\/revisions"}],"predecessor-version":[{"id":1607,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages\/312\/revisions\/1607"}],"up":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages\/199"}],"wp:attachment":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/media?parent=312"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}