{"id":152,"date":"2018-01-08T12:46:44","date_gmt":"2018-01-08T12:46:44","guid":{"rendered":"http:\/\/confolab.sav.sk\/ovsb\/en\/?page_id=152"},"modified":"2024-02-16T14:09:11","modified_gmt":"2024-02-16T13:09:11","slug":"cell-culture","status":"publish","type":"page","link":"https:\/\/confolab.sav.sk\/ovsb\/en\/laboratories\/cell-culture\/","title":{"rendered":"Cell Culture"},"content":{"rendered":"<h2>CO2 incubator (ESCO), Class II biosafety cabinet (ESCO), steam autoclave (IcanClav)<br \/>\n&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<button onclick=\"goBack()\">Back<\/button> <\/h2>\n<p><script>\t \t \nfunction goBack() { window.history.go(-1); }\t \t \n<\/script><\/p>\n<h3>Contact: RNDr. Michal Cagalinec, PhD.<\/h3>\n<h3>Short term culture of primary isolated left ventricular myocytes and cortical neurons and transfection using:<\/h3>\n<ul>\n<li>Lipofectamine2000\u00ae<\/li>\n<li>adenoviruses<\/li>\n<\/ul>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-1009\" src=\"http:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/DSC_1393_2-293x300.jpg\" alt=\"DSC_1393_2\" width=\"139\" height=\"142\" \/><\/td>\n<td><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-1010\" src=\"http:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/DSC_1105_1-300x247.jpg\" alt=\"DSC_1105_1\" width=\"173\" height=\"142\" \/><\/td>\n<td><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-1012\" src=\"http:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/DSC_1315_2-300x235.jpg\" alt=\"DSC_1315_2\" width=\"181\" height=\"142\" \/><\/td>\n<td><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-1011\" src=\"http:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/DSC_1381_1-300x264.jpg\" alt=\"DSC_1381_1\" width=\"160\" height=\"141\" \/><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Main focus of the lab is analysis of dynamic remodeling of organelles and pathophysiological processes in cardiac myocytes and neurons using fluorescent indicators and proteins, where fluorescence is detected by STED, confocal and fluorescence microscopy.<\/p>\n<p>To express fluorescent proteins in the myocytes and\/or neurons, circular bacterial plasmids have to be transfected into the cytoplasm either by lipofection (Lipofectamine2000\u00ae) or by adenoviral transduction.<\/p>\n<h3>Recently we plan to express:<\/h3>\n<ul>\n<li>MitoKikumeGR1 \u2013 photoconvertible protein (green \u2192 red) targeted to mitochondria \u2013 fusion-fission dynamics in myocytes<\/li>\n<li>Perceval \u2013 fluorescent FRET protein (CFP-YFP) \u2013 to estimate the ratio of ATP\/ADP in the cell<\/li>\n<li>Chameleon D3cpv \u2013 fluorescent FRET protein (CFP-YFP) \u2013 cytoplasmic, mitochondrial and ER\/SR [Ca2+] measurement<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>CO2 incubator (ESCO), Class II biosafety cabinet (ESCO), steam autoclave (IcanClav) &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Back Contact: RNDr. Michal Cagalinec, PhD. Short term culture of primary isolated left ventricular&#8230;<span class=\"excerpt_more\"><a href=\"https:\/\/confolab.sav.sk\/ovsb\/en\/laboratories\/cell-culture\/\">\u010d\u00edta\u0165 \u010falej &raquo;<\/a><\/span><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":42,"menu_order":80,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages\/152"}],"collection":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/comments?post=152"}],"version-history":[{"count":4,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages\/152\/revisions"}],"predecessor-version":[{"id":1636,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages\/152\/revisions\/1636"}],"up":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages\/42"}],"wp:attachment":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/media?parent=152"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}