{"id":148,"date":"2018-01-08T12:46:44","date_gmt":"2018-01-08T12:46:44","guid":{"rendered":"http:\/\/confolab.sav.sk\/ovsb\/en\/?page_id=148"},"modified":"2021-02-25T11:05:37","modified_gmt":"2021-02-25T10:05:37","slug":"patch-clamp","status":"publish","type":"page","link":"https:\/\/confolab.sav.sk\/ovsb\/en\/laboratories\/patch-clamp\/","title":{"rendered":"Patch clamp"},"content":{"rendered":"<h2><span class=\"H3\">Patch clamp amplifiers Axopatch 200-B (Axon Instruments), VE-2 (Alembic Instruments), stimulator IonOptix MYOPACER, micropipette puller P-97 (Sutter Instruments) <\/span>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<button onclick=\"goBack()\">Back<\/button> <\/h2>\n<p><script>\t \t \nfunction goBack() { window.history.go(-1); }\t \t \n<\/script><\/p>\n<h3>Contact: RNDr. Ivan Zahradn\u00edk, CSc.<\/h3>\n<p><b>Patch clamp<\/b> is used primarily for measurements of<\/p>\n<ul style=\"line-height:1.3\">\n<li>calcium currents in living isolated cardiac myocytes using the voltage clamp method<\/li>\n<li>application of indicators, buffers and metabolites into the cytosol of cardiac myocytes<\/li>\n<li>stimulation of cardiac myocytes to evoke local calcium release<\/li>\n<li>measurement of passive electrical properties of cells (membrane capacitance and membrane resistance) with high sensitivity and temporal resolution<\/li>\n<\/ul>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td><img decoding=\"async\" loading=\"lazy\" class=\"wp-image-555 aligncenter\" src=\"http:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/perfusion-300x199-300x199.jpg\" alt=\"perfusion\" width=\"206\" height=\"137\" \/><\/td>\n<td><img decoding=\"async\" loading=\"lazy\" class=\"wp-image-556 aligncenter\" src=\"http:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/patch-clamp-300x198-300x198.jpg\" alt=\"patch clamp\" width=\"207\" height=\"137\" \/><\/td>\n<td><img decoding=\"async\" loading=\"lazy\" class=\"wp-image-562 aligncenter\" src=\"http:\/\/confolab.sav.sk\/ovsb\/wp-content\/uploads\/cell3-300x198-300x198.png\" alt=\"cell3\" width=\"206\" height=\"136\" \/><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"line-height:1.3\">We are interested in the modulation of activation and inactivation of calcium currents through L-type calcium channels under different conditions, and i the changes of calcium currents evoked by physiological and pathological stimuli.<\/p>\n<p style=\"line-height:1.3\">The method of <b>&#8220;field stimulation&#8221;<\/b> is used to stimulate cells with external electrodes. We are interested in changes of calcium release and contractility evoked by physiological and pathological stimuli.<\/p>\n<p style=\"line-height:1.3\">Measurement of <b>membrane capacitance with high resolution<\/b> is used as a tool to study the dynamics of surface membrane and t-tubules in cardiac myocytes.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Patch clamp amplifiers Axopatch 200-B (Axon Instruments), VE-2 (Alembic Instruments), stimulator IonOptix MYOPACER, micropipette puller P-97 (Sutter Instruments) &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Back Contact: RNDr. Ivan Zahradn\u00edk, CSc. Patch&#8230;<span class=\"excerpt_more\"><a href=\"https:\/\/confolab.sav.sk\/ovsb\/en\/laboratories\/patch-clamp\/\">\u010d\u00edta\u0165 \u010falej &raquo;<\/a><\/span><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":42,"menu_order":40,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages\/148"}],"collection":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/comments?post=148"}],"version-history":[{"count":6,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages\/148\/revisions"}],"predecessor-version":[{"id":1183,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages\/148\/revisions\/1183"}],"up":[{"embeddable":true,"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/pages\/42"}],"wp:attachment":[{"href":"https:\/\/confolab.sav.sk\/ovsb\/en\/wp-json\/wp\/v2\/media?parent=148"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}